NAD+ (Nicotinamide adenine dinucleotide) is a coenzyme with a molecular formula of C21H27N7O14P2 and a molar mass of 663.43 g/mol. As a research-grade material, high-purity NAD+ is essential for accurate laboratory analysis, with a typical purity of ≥98% as confirmed by HPLC. For optimal storage, NAD+ should be kept at -20°C to preserve its stability and prevent degradation. Researchers can source research-grade NAD+ from reputable suppliers, such as Alpha Amino, which provides a per-lot Certificate of Analysis to ensure the quality and authenticity of the material.
Molecular and Structural Properties
NAD+ is a dinucleotide coenzyme consisting of two nucleotides, nicotinamide mononucleotide (NMN) and adenosine monophosphate (AMP), linked by a pyrophosphate bond. Its molecular structure is characterized by a planar, aromatic ring system, with a nicotinamide moiety and an adenine base. The coenzyme plays a crucial role in various biochemical reactions, including redox reactions, and is an essential component of many enzyme-catalyzed reactions.
Physical Characteristics and Appearance
NAD+ is a white to off-white crystalline powder with a characteristic UV absorption spectrum. Its solubility in water is relatively high, making it suitable for preparation of stock solutions for laboratory analysis. The coenzyme is also relatively stable in aqueous solutions, but its stability can be affected by factors such as pH, temperature, and the presence of divalent metal ions.
Purity and Quality Verification
To ensure the quality and authenticity of NAD+ for research purposes, it is essential to verify its purity and identity. HPLC (High-Performance Liquid Chromatography) analysis is commonly used to determine the purity of NAD+, with a minimum purity of ≥98% required for research-grade material. Mass spectrometry can also be used to confirm the identity of NAD+ by its molecular weight and fragmentation pattern. A per-lot Certificate of Analysis, such as that provided by Alpha Amino, is a critical document that certifies the quality and purity of the material.
| Property |
Value |
| Molecular Formula |
C21H27N7O14P2 |
| Molar Mass |
663.43 g/mol |
| Purity |
≥98% (HPLC) |
| Solubility |
Highly soluble in water |
| Storage Temperature |
-20°C |
Preparation of Research Stock Solutions
For laboratory analysis, NAD+ stock solutions can be prepared by dissolving the coenzyme in water or a suitable buffer solution. The concentration of the stock solution will depend on the specific requirements of the experiment, but a typical concentration range is 10-100 mM. To maintain the stability of the coenzyme, it is essential to handle the stock solution aseptically and store it at -20°C.
Storage and Stability
NAD+ is a relatively stable coenzyme, but its stability can be affected by factors such as temperature, humidity, and light. To preserve its stability, NAD+ should be stored at -20°C in a dry, dark place. The coenzyme is also sensitive to moisture, and exposure to air should be minimized to prevent degradation.
Selecting a Research-Grade Source
When selecting a research-grade source of NAD+, it is essential to verify the quality and authenticity of the material. Researchers should look for a supplier that provides a per-lot Certificate of Analysis, HPLC purity confirmation, and mass spectrometry identity confirmation. Additionally, the supplier should be based in the US and provide transparent lot numbers. Alpha Amino is a reputable supplier of research-grade compounds, including NAD+, and provides a per-lot Certificate of Analysis to ensure the quality and authenticity of the material. For specific information on NAD+ products, researchers can visit the research-grade compounds page.
Frequently asked questions
What is the typical purity of research-grade NAD+?
The typical purity of research-grade NAD+ is ≥98% as confirmed by HPLC. This high level of purity is essential for accurate laboratory analysis and ensures the reliability of experimental results. Researchers should always verify the purity of NAD+ by checking the Certificate of Analysis provided by the supplier.
How should NAD+ be stored to preserve its stability?
NAD+ should be stored at -20°C in a dry, dark place to preserve its stability. The coenzyme is sensitive to moisture, and exposure to air should be minimized to prevent degradation. Researchers should also handle the stock solution aseptically to maintain its stability.
What is the importance of a per-lot Certificate of Analysis for NAD+?
A per-lot Certificate of Analysis is a critical document that certifies the quality and purity of NAD+. It provides confirmation of the coenzyme’s identity, purity, and concentration, ensuring that the material meets the required standards for research-grade material. Researchers should always verify the Certificate of Analysis before purchasing NAD+ from a supplier.
How can researchers verify the quality of NAD+ from a supplier?
Researchers can verify the quality of NAD+ from a supplier by checking the Certificate of Analysis, HPLC purity confirmation, and mass spectrometry identity confirmation. They should also look for a supplier that provides transparent lot numbers and is based in the US. Alpha Amino is a reputable supplier of research-grade compounds, including NAD+, and provides a per-lot Certificate of Analysis to ensure the quality and authenticity of the material.
What is the typical concentration range of NAD+ stock solutions for laboratory analysis?
The typical concentration range of NAD+ stock solutions for laboratory analysis is 10-100 mM. However, the concentration of the stock solution will depend on the specific requirements of the experiment, and researchers should consult the relevant protocols and guidelines for the specific application.
Alpha Amino products are sold strictly for laboratory and research use only and are not intended for human or veterinary use, diagnosis, treatment, or consumption.
