Bacteriostatic water, with a molecular formula of H2O and a molar mass of 18.015 g/mol, is a crucial component in various in vitro laboratory applications, including the preparation of research stock solutions. To maintain its chemical stability and shelf life, it is essential to store bacteriostatic water at a temperature range of 2-8°C, with a pH level between 5.5 and 7.0. The addition of 0.1% benzyl alcohol as a bacteriostatic agent helps prevent microbial growth, ensuring the water remains sterile and suitable for laboratory use.
Chemical Identity and Classification
Bacteriostatic water is a type of water that has been treated with a bacteriostatic agent to prevent the growth of microorganisms. It is commonly used in laboratory settings as a solvent or diluent for various research applications. The chemical identity of bacteriostatic water is characterized by its molecular formula and molar mass, which are identical to those of regular water. However, the addition of a bacteriostatic agent sets it apart from other types of water.
Molecular and Structural Properties
The molecular structure of bacteriostatic water is composed of two hydrogen atoms and one oxygen atom, with a bent or V-shape configuration. The addition of benzyl alcohol as a bacteriostatic agent does not alter the molecular structure of the water itself but rather inhibits the growth of microorganisms. The pH level of bacteriostatic water is a critical factor in maintaining its chemical stability and shelf life, as extreme pH levels can lead to degradation or contamination of the water.
Physical Characteristics
Bacteriostatic water is a clear, colorless liquid with a density of approximately 1 g/ml. It has a boiling point of 100°C and a freezing point of 0°C, similar to regular water. The physical characteristics of bacteriostatic water make it an ideal solvent or diluent for various laboratory applications, including the preparation of research stock solutions.
Purity and Quality Verification
To ensure the purity and quality of bacteriostatic water, it is essential to verify its chemical composition and sterility. High-performance liquid chromatography (HPLC) and mass spectrometry can be used to confirm the absence of contaminants and impurities. A per-lot Certificate of Analysis, such as those provided by research-grade compound suppliers like Alpha Amino, can also provide assurance of the water’s quality and purity.
| Parameter |
Specification |
| pH Level |
5.5-7.0 |
| Conductivity |
<1.3 μS/cm |
| TOC (Total Organic Carbon) |
<50 ppb |
| Bacterial Endotoxins |
<0.06 EU/ml |
Selecting and Verifying a Research-Grade Source
When selecting a research-grade source of bacteriostatic water, it is crucial to verify the quality and purity of the product. Look for suppliers that provide a per-lot Certificate of Analysis, HPLC purity confirmation, and mass-spectrometry identity confirmation. Additionally, ensure that the supplier is based in the US and provides transparent lot numbers. Research-grade bacteriostatic water from reputable suppliers like Alpha Amino can provide the necessary assurance of quality and purity for in vitro laboratory applications.
Storage and Stability
To maintain the chemical stability and shelf life of bacteriostatic water, it is essential to store it in a cool, dry place, away from direct sunlight and moisture. The water should be kept in a tightly sealed container, such as a glass bottle with a screw-cap lid, to prevent contamination. The storage temperature should be between 2-8°C, and the water should be used within a specified period, typically 12-18 months from the date of manufacture.
Frequently asked questions
What is the recommended storage temperature for bacteriostatic water?
The recommended storage temperature for bacteriostatic water is between 2-8°C. This temperature range helps maintain the chemical stability and shelf life of the water. It is essential to store the water in a cool, dry place, away from direct sunlight and moisture.
How can I verify the purity and quality of bacteriostatic water?
To verify the purity and quality of bacteriostatic water, you can use HPLC and mass spectrometry to confirm the absence of contaminants and impurities. Additionally, look for a per-lot Certificate of Analysis from the supplier, which provides assurance of the water’s quality and purity.
What are the critical factors in maintaining the chemical stability and shelf life of bacteriostatic water?
The critical factors in maintaining the chemical stability and shelf life of bacteriostatic water include storing it at a temperature range of 2-8°C, maintaining a pH level between 5.5 and 7.0, and preventing contamination. It is also essential to use a tightly sealed container and keep the water away from direct sunlight and moisture.
How can I select a reputable supplier of research-grade bacteriostatic water?
To select a reputable supplier of research-grade bacteriostatic water, look for suppliers that provide a per-lot Certificate of Analysis, HPLC purity confirmation, and mass-spectrometry identity confirmation. Additionally, ensure that the supplier is based in the US and provides transparent lot numbers. Suppliers like Alpha Amino can provide the necessary assurance of quality and purity for in vitro laboratory applications.
What is the importance of using bacteriostatic water in laboratory applications?
Bacteriostatic water is essential in laboratory applications as it provides a sterile and stable solvent or diluent for various research purposes. The use of bacteriostatic water helps prevent microbial growth and contamination, ensuring the accuracy and reliability of laboratory results.
Alpha Amino products are sold strictly for laboratory and research use only and are not intended for human or veterinary use, diagnosis, treatment, or consumption.
