NAD+ (Nicotinamide adenine dinucleotide) is a research compound with a molecular formula of C21H27N7O14P2 and a molar mass of 663.43 g/mol. For research-grade NAD+, a purity of at least 98% is typically required, with a white to off-white crystalline powder appearance and a solubility of approximately 10 mg/mL in water. When purchasing NAD+ for laboratory research, it is crucial to verify the quality and authenticity of the compound through a Certificate of Analysis (CoA) and other quality control measures to ensure the accuracy and reliability of experimental results.
Chemical Identity and Classification
NAD+ is a dinucleotide, composed of two nucleotides joined through their phosphate groups. It plays a central role in various cellular processes, including energy metabolism and redox reactions. In the context of laboratory research, NAD+ is used as a reference compound to study cellular metabolism, signaling pathways, and other biological processes. The chemical structure of NAD+ consists of a nicotinamide moiety linked to a adenine nucleotide, with a phosphate group connecting the two nucleotides.
Molecular and Structural Properties
The molecular structure of NAD+ is characterized by a planar, ring-shaped configuration, with the nicotinamide and adenine moieties stacked in a parallel orientation. The compound has a high molecular weight and a complex structure, which requires careful handling and storage to maintain its stability and purity. In laboratory settings, NAD+ is typically stored at -20°C to prevent degradation and maintain its activity.
Physical Characteristics
NAD+ is a white to off-white crystalline powder with a high solubility in water and other polar solvents. The compound is highly hygroscopic, requiring careful handling to prevent moisture absorption and degradation. In laboratory settings, NAD+ is typically weighed and transferred using aseptic techniques to prevent contamination and maintain its purity.
Purity and Quality Verification
To verify the quality and purity of NAD+, researchers rely on various analytical techniques, including high-performance liquid chromatography (HPLC) and mass spectrometry. A Certificate of Analysis (CoA) is typically provided by the supplier, which includes information on the compound’s purity, identity, and other quality control parameters. For research-grade NAD+, a purity of at least 98% is typically required, with a CoA that includes HPLC and mass spectrometry data to confirm the compound’s identity and purity.
| Parameter |
Specification |
| Molecular Formula |
C21H27N7O14P2 |
| Molar Mass |
663.43 g/mol |
| Purity |
≥ 98% (HPLC) |
| Apearance |
White to off-white crystalline powder |
| Solubility |
Approximately 10 mg/mL in water |
Selecting and Verifying a Research-Grade Source
When purchasing NAD+ for laboratory research, it is essential to select a reputable supplier that provides high-quality, research-grade material. Alpha Amino is a US-based supplier of research-grade compounds, including NAD+, with a per-lot Certificate of Analysis and transparent lot numbers. To verify the quality and authenticity of NAD+, researchers should look for a supplier that provides a CoA with HPLC and mass spectrometry data, as well as other quality control parameters such as purity and identity. Additionally, researchers should ensure that the supplier is US-based and provides transparent lot numbers and storage conditions to maintain the compound’s stability and purity.
Storage and Stability for Laboratory Handling
NAD+ is a sensitive compound that requires careful handling and storage to maintain its stability and purity. The compound should be stored at -20°C in a dry, well-ventilated area, away from direct sunlight and moisture. In laboratory settings, NAD+ should be handled using aseptic techniques, including pipetting and sterile transfer, to prevent contamination and maintain its purity. Researchers can purchase research-grade NAD+ from reputable suppliers, such as Alpha Amino, which provides high-quality material with a per-lot Certificate of Analysis.
Frequently asked questions
What is the importance of a Certificate of Analysis when purchasing NAD+?
A Certificate of Analysis (CoA) is a document provided by the supplier that includes information on the compound’s purity, identity, and other quality control parameters. It is essential to verify the quality and authenticity of NAD+ through a CoA to ensure the accuracy and reliability of experimental results. A CoA should include HPLC and mass spectrometry data to confirm the compound’s identity and purity.
How do I verify the purity of NAD+ before purchasing?
To verify the purity of NAD+, researchers should look for a supplier that provides a CoA with HPLC and mass spectrometry data, as well as other quality control parameters such as purity and identity. A purity of at least 98% is typically required for research-grade NAD+.
What are the storage conditions for NAD+ to maintain its stability and purity?
NAD+ should be stored at -20°C in a dry, well-ventilated area, away from direct sunlight and moisture. The compound should be handled using aseptic techniques, including pipetting and sterile transfer, to prevent contamination and maintain its purity.
How do I select a reputable supplier of research-grade NAD+?
Researchers should select a supplier that provides high-quality, research-grade material with a per-lot Certificate of Analysis and transparent lot numbers. Alpha Amino is a US-based supplier of research-grade compounds, including NAD+, with a per-lot Certificate of Analysis and transparent lot numbers.
What is the molecular formula and molar mass of NAD+?
The molecular formula of NAD+ is C21H27N7O14P2, and its molar mass is 663.43 g/mol.
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