BPC-157, also known as Body Protection Compound 157, is a synthetic peptide with the amino acid sequence GEPPGDGAEGGPGPAG, a molar mass of approximately 1419.55 g/mol, and a purity of at least 98% as verified by HPLC. This research-grade compound is typically provided as a white lyophilized powder, soluble in sterile water or bacteriostatic water, with a recommended storage temperature of -20°C. For research purposes, BPC-157 is often prepared as a stock solution in aseptic conditions, utilizing sterile pipetting and transfer techniques to maintain the integrity of the sample.
Chemical Identity and Classification
BPC-157 is a pentadecapeptide, consisting of 15 amino acids, with a molecular formula of C62H98N16O22. Its chemical structure and properties make it an important reference compound in various in-vitro studies. The compound’s identity can be confirmed through mass spectrometry and HPLC analysis, which are essential techniques for verifying the purity and quality of BPC-157.
Molecular and Structural Properties
The molecular structure of BPC-157 is characterized by its specific sequence of amino acids, which determines its physical and chemical properties. The peptide’s solubility, stability, and reactivity are influenced by its molecular weight, charge, and hydrophobicity. Understanding these properties is crucial for handling and preparing BPC-157 for laboratory research.
Physical Characteristics and Handling
BPC-157 is typically provided in a lyophilized form, which requires careful handling and storage to maintain its quality. The powder should be stored at -20°C, protected from light and moisture, and handled using aseptic techniques to prevent contamination. When preparing a stock solution, sterile water or bacteriostatic water should be used, and the solution should be filtered and transferred using sterile pipettes and equipment.
Purity and Quality Verification
The purity and quality of BPC-157 can be verified through various analytical techniques, including HPLC, mass spectrometry, and amino acid analysis. A per-lot Certificate of Analysis (COA) should be provided by the supplier, detailing the compound’s purity, identity, and other relevant specifications. Research-grade BPC-157 should have a purity of at least 98%, as confirmed by HPLC analysis.
| Specification |
Value |
| Molecular Formula |
C62H98N16O22 |
| Molar Mass |
approximately 1419.55 g/mol |
| Purity |
>= 98% (HPLC) |
| Appearance |
White lyophilized powder |
| Solubility |
Soluble in sterile water or bacteriostatic water |
Selecting and Verifying a Research-Grade Source
When selecting a supplier of research-grade BPC-157, it is essential to verify the quality and authenticity of the compound. Look for a supplier that provides a per-lot Certificate of Analysis, detailing the compound’s purity, identity, and other relevant specifications. Additionally, ensure that the supplier is based in the US and provides research-grade material, such as research-grade compounds from Alpha Amino. A reputable supplier should be transparent about their manufacturing and quality control processes, and provide clear instructions for handling and storage.
Storage and Stability for Laboratory Handling
BPC-157 should be stored at -20°C, protected from light and moisture, to maintain its stability and quality. The compound’s lyophilized form is stable for several months when stored properly, but it is recommended to prepare a stock solution immediately before use. When handling BPC-157, aseptic techniques should be used to prevent contamination, and the compound should be transferred using sterile pipettes and equipment.
Frequently asked questions
What is the recommended storage temperature for BPC-157?
The recommended storage temperature for BPC-157 is -20°C, protected from light and moisture. This will help maintain the stability and quality of the compound. It is also essential to handle the compound using aseptic techniques and store it in a sealed container to prevent contamination.
How can I verify the purity and quality of BPC-157?
The purity and quality of BPC-157 can be verified through various analytical techniques, including HPLC, mass spectrometry, and amino acid analysis. A per-lot Certificate of Analysis (COA) should be provided by the supplier, detailing the compound’s purity, identity, and other relevant specifications. Look for a supplier that provides research-grade material, such as Alpha Amino, which offers research-grade compounds with a per-lot COA.
What is the importance of a per-lot Certificate of Analysis?
A per-lot Certificate of Analysis (COA) is essential for verifying the purity and quality of BPC-157. The COA provides detailed information about the compound’s purity, identity, and other relevant specifications, ensuring that the researcher receives a high-quality compound. A reputable supplier, such as Alpha Amino, should provide a per-lot COA for each batch of BPC-157.
How can I prepare a stock solution of BPC-157?
A stock solution of BPC-157 can be prepared by dissolving the lyophilized powder in sterile water or bacteriostatic water. The solution should be filtered and transferred using sterile pipettes and equipment to prevent contamination. It is recommended to prepare the stock solution immediately before use and to store it at -20°C to maintain its stability and quality.
What should I look for in a supplier of research-grade BPC-157?
When selecting a supplier of research-grade BPC-157, look for a supplier that provides a per-lot Certificate of Analysis, detailing the compound’s purity, identity, and other relevant specifications. Additionally, ensure that the supplier is based in the US and provides research-grade material, such as Alpha Amino. A reputable supplier should be transparent about their manufacturing and quality control processes, and provide clear instructions for handling and storage.
Alpha Amino products are sold strictly for laboratory and research use only and are not intended for human or veterinary use, diagnosis, treatment, or consumption.
