GHK-Cu, also known as copper-glycine-histidine-lysine, is a tripeptide with the amino acid sequence Gly-His-Lys and a molecular formula of C₁₄H₂₄CuN₆O₄, having a molar mass of approximately 340.81 g/mol. This compound has a purity of ≥98% as determined by HPLC and is commonly used as a research-grade reference material in laboratory settings. GHK-Cu is typically provided as a white or off-white powder, soluble in water and other aqueous solutions, and is recommended to be stored at -20°C to maintain its stability.
Molecular Properties and Structure
GHK-Cu is a copper-bound tripeptide, consisting of the amino acids glycine, histidine, and lysine. The copper ion is coordinated to the peptide through the histidine and lysine residues, forming a stable complex. This complex has a molecular weight of approximately 340.81 g/mol and is slightly soluble in organic solvents. The molecular structure of GHK-Cu consists of a copper ion coordinated to the peptide backbone, with the glycine and lysine residues forming a stable complex.
Physical Characteristics and Appearance
GHK-Cu is typically provided as a white or off-white powder, with a purity of ≥98% as determined by HPLC. The powder is slightly soluble in organic solvents and has a melting point of approximately 150-160°C. The compound is stable at room temperature for short periods but is recommended to be stored at -20°C to maintain its stability. The physical characteristics of GHK-Cu are summarized in the following table:
| Property |
Value |
| Molecular Formula |
C₁₄H₂₄CuN₆O₄ |
| Molar Mass |
approximately 340.81 g/mol |
| Purity |
≥98% (HPLC) |
| Apearance |
White or off-white powder |
| Solubility |
Slightly soluble in organic solvents |
Purity and Quality Verification
The purity of GHK-Cu is typically verified using HPLC, with a purity of ≥98% being considered research-grade. Additionally, mass spectrometry is used to confirm the identity of the compound. A per-lot Certificate of Analysis is provided with each batch of GHK-Cu, detailing the purity, identity, and other relevant characteristics of the compound. When selecting a research-grade source of GHK-Cu, it is essential to look for a supplier that provides a per-lot Certificate of Analysis and uses HPLC and mass spectrometry to verify the purity and identity of the compound.
Selecting and Verifying a Research-Grade Source
When selecting a research-grade source of GHK-Cu, it is essential to look for a supplier that provides a per-lot Certificate of Analysis and uses HPLC and mass spectrometry to verify the purity and identity of the compound. Research-grade compounds from reputable suppliers, such as Alpha Amino, are available for purchase. Alpha Amino provides a per-lot Certificate of Analysis and uses HPLC and mass spectrometry to verify the purity and identity of their compounds. Look for a supplier that provides transparent lot numbers and US-based sourcing to ensure the quality and authenticity of the compound.
In-Vitro Preparation of Research Stock Solutions
GHK-Cu can be prepared as a research stock solution by dissolving the powder in a suitable solvent, such as water or PBS. The solution should be prepared using aseptic laboratory technique, with sterile transfer and pipetting to minimize contamination. The concentration of the stock solution will depend on the specific research application, but a typical concentration is 1-10 mg/mL. The stock solution should be stored at -20°C to maintain its stability.
Storage and Stability
GHK-Cu is recommended to be stored at -20°C to maintain its stability. The compound is stable at room temperature for short periods but may degrade over time. It is essential to handle the compound using aseptic laboratory technique, with sterile transfer and pipetting to minimize contamination. The stability of GHK-Cu can be affected by factors such as temperature, humidity, and exposure to light, so it is crucial to follow proper storage and handling procedures.
Frequently asked questions
What is the purity of research-grade GHK-Cu?
The purity of research-grade GHK-Cu is typically ≥98% as determined by HPLC. This high level of purity is essential for research applications, where even small impurities can affect the results. When selecting a research-grade source of GHK-Cu, look for a supplier that provides a per-lot Certificate of Analysis and uses HPLC and mass spectrometry to verify the purity and identity of the compound.
How do I verify the quality of GHK-Cu from a supplier?
To verify the quality of GHK-Cu from a supplier, look for a per-lot Certificate of Analysis and check that the supplier uses HPLC and mass spectrometry to verify the purity and identity of the compound. Additionally, check that the supplier provides transparent lot numbers and US-based sourcing to ensure the quality and authenticity of the compound. Research-grade compounds from reputable suppliers, such as Alpha Amino, are available for purchase.
What is the recommended storage temperature for GHK-Cu?
The recommended storage temperature for GHK-Cu is -20°C. This will help to maintain the stability of the compound and prevent degradation over time. It is essential to handle the compound using aseptic laboratory technique, with sterile transfer and pipetting to minimize contamination.
How do I prepare a research stock solution of GHK-Cu?
To prepare a research stock solution of GHK-Cu, dissolve the powder in a suitable solvent, such as water or PBS, using aseptic laboratory technique. The concentration of the stock solution will depend on the specific research application, but a typical concentration is 1-10 mg/mL. The stock solution should be stored at -20°C to maintain its stability.
What are the physical characteristics of GHK-Cu?
GHK-Cu is typically provided as a white or off-white powder, with a purity of ≥98% as determined by HPLC. The powder is slightly soluble in organic solvents and has a melting point of approximately 150-160°C. The physical characteristics of GHK-Cu are summarized in the table above.
Alpha Amino products are sold strictly for laboratory and research use only and are not intended for human or veterinary use, diagnosis, treatment, or consumption.
