Bacteriostatic water, with a chemical formula of H2O and a molar mass of approximately 18.02 g/mol, is a crucial component in various in-vitro laboratory applications, including the preparation of research stock solutions and the dilution of reference compounds. Research-grade bacteriostatic water products, such as those available from research-grade compounds suppliers, typically have a purity of at least 99% and contain 0.1% benzyl alcohol as a bacteriostatic agent. To ensure optimal in-vitro laboratory performance, it is essential to select a high-quality bacteriostatic water product with a per-lot Certificate of Analysis, transparent lot numbers, and US-based sourcing.
Chemical Identity and Classification
Bacteriostatic water is a type of sterile water that contains a bacteriostatic agent, typically benzyl alcohol, to prevent the growth of microorganisms. The chemical identity of bacteriostatic water is characterized by its molecular formula, H2O, and its CAS number, 7732-18-5. The bacteriostatic agent, benzyl alcohol, has a molecular formula of C6H5CH2OH and a CAS number of 100-51-6.
Molecular and Structural Properties
The molecular structure of bacteriostatic water is similar to that of sterile water, with the addition of benzyl alcohol molecules. The benzyl alcohol molecules are dispersed throughout the water, providing a bacteriostatic effect. The molecular weight of bacteriostatic water is approximately 18.02 g/mol, which is similar to that of sterile water.
Physical Characteristics
Research-grade bacteriostatic water products are typically clear, colorless liquids with a pH range of 5.5-7.5. The appearance of bacteriostatic water is similar to that of sterile water, with no visible particles or contaminants. The physical characteristics of bacteriostatic water are critical in ensuring optimal in-vitro laboratory performance.
Purity and Quality Verification
The purity and quality of research-grade bacteriostatic water products are verified through various analytical techniques, including HPLC and mass spectrometry. A per-lot Certificate of Analysis is typically provided with each batch of bacteriostatic water, detailing the purity, pH, and other critical parameters. The following table compares the specifications of different research-grade bacteriostatic water products:
| Parameter |
Product A |
Product B |
Product C |
| Purity |
>99% |
>98% |
>99.5% |
| pH |
5.5-7.5 |
5.0-7.0 |
5.5-7.5 |
| Benzyl Alcohol Concentration |
0.1% |
0.1% |
0.1% |
Selecting and Verifying a Research-Grade Source
When selecting a research-grade bacteriostatic water product, it is essential to verify the quality markers, including a per-lot Certificate of Analysis, HPLC purity, mass-spectrometry identity confirmation, transparent lot numbers, and US-based sourcing. Alpha Amino, a US-based supplier of research-grade compounds, provides high-quality bacteriostatic water products with a per-lot Certificate of Analysis and transparent lot numbers. Researchers should look for suppliers that provide detailed product specifications, including purity, pH, and benzyl alcohol concentration, to ensure optimal in-vitro laboratory performance.
Storage and Stability for Laboratory Handling
Research-grade bacteriostatic water products should be stored in a cool, dry place, away from direct sunlight and moisture. The recommended storage temperature is between 2-8°C, and the product should be used within the expiration date specified on the label. When handling bacteriostatic water, it is essential to use aseptic laboratory technique, including pipetting and sterile transfer, to prevent contamination.
Frequently Asked Questions
What is the difference between bacteriostatic water and sterile water?
Bacteriostatic water contains a bacteriostatic agent, typically benzyl alcohol, to prevent the growth of microorganisms, whereas sterile water does not contain any additives. Bacteriostatic water is typically used in in-vitro laboratory applications where the prevention of microbial growth is critical.
How do I verify the purity of a bacteriostatic water product?
The purity of a bacteriostatic water product can be verified through various analytical techniques, including HPLC and mass spectrometry. A per-lot Certificate of Analysis is typically provided with each batch of bacteriostatic water, detailing the purity, pH, and other critical parameters.
What are the critical parameters to consider when selecting a research-grade bacteriostatic water product?
The critical parameters to consider when selecting a research-grade bacteriostatic water product include purity, pH, benzyl alcohol concentration, and the presence of a per-lot Certificate of Analysis. Researchers should also consider the supplier’s reputation, transparency, and US-based sourcing.
How should I store and handle bacteriostatic water products?
Bacteriostatic water products should be stored in a cool, dry place, away from direct sunlight and moisture. The recommended storage temperature is between 2-8°C, and the product should be used within the expiration date specified on the label. When handling bacteriostatic water, it is essential to use aseptic laboratory technique, including pipetting and sterile transfer, to prevent contamination.
What is the recommended source for research-grade bacteriostatic water products?
Alpha Amino, a US-based supplier of research-grade compounds, provides high-quality bacteriostatic water products with a per-lot Certificate of Analysis and transparent lot numbers. Researchers should consider suppliers that provide detailed product specifications, including purity, pH, and benzyl alcohol concentration, to ensure optimal in-vitro laboratory performance.
Alpha Amino products are sold strictly for laboratory and research use only and are not intended for human or veterinary use, diagnosis, treatment, or consumption.
